Anti-SUR1/ABCC8 Antibody Picoband™

Product Name

Anti-SUR1/ABCC8 Antibody Picoband™

View all SUR1 Antibodies

SKU/Catalog Number

A00895-1

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SUR1/ABCC8 Antibody Picoband™ catalog # A00895-1. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SUR1/ABCC8 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00895-1)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human SUR1, which shares 97.7% amino acid (aa) sequence identity with both mouse and rat SUR1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00895-1 is reactive to ABCC8 in Human, Mouse, Rat

Applications

A00895-1 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

177 kDa

Calculated molecular weight

176.992kDa

Background of SUR1

ATP-binding cassette transporter sub-family C member 8 is a protein that in humans is encoded by the ABCC8 gene. The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions as a modulator of ATP-sensitive potassium channels and insulin release. Mutations and deficiencies in this protein have been observed in patients with hyperinsulinemic hypoglycemia of infancy, an autosomal recessive disorder of unregulated and high insulin secretion. Mutations have also been associated with non-insulin-dependent diabetes mellitus type II, an autosomal dominant disease of defective insulin secretion. Alternatively spliced transcript variants have been found for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x10⁶ cells

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of SUR1 using anti-SUR1 antibody (A00895-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SUR1 antigen affinity purified polyclonal antibody (Catalog # A00895-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SUR1 at approximately 177KD. The expected band size for SUR1 is at 177KD.

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Figure 2. Flow Cytometry analysis of A431 cells using anti-ABCC8 antibody (A00895-1).
Overlay histogram showing A431 cells stained with A00895-1 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCC8 Antibody (A00895-1,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For ABCC8 (Source: Uniprot.org, NCBI)

Alternative Names

ABC36; ATP-binding cassette, sub-family C (CFTR/MRP), member 8; HHF1Sulfonylurea receptor 1; HI; PHHIHRINS; sulfonylurea receptor (hyperinsulinemia); SUR1MRP8; SURATP-binding cassette transporter sub-family C member 8; TNDM2ATP-binding cassette sub-family C member 8 ABCC8 ABC36, HHF1, HI, HRINS, MRP8, PHHI, PNDM3, SUR, SUR1, SUR1delta2, TNDM2 ATP binding cassette subfamily C member 8 ATP-binding cassette sub-family C member 8|ATP-binding cassette transporter sub-family C member 8|ATP-binding cassette, sub-family C (CFTR/MRP), member 8|sulfonylurea receptor (hyperinsulinemia)|sulfonylurea receptor 1

For more info on ABCC8, check out the ABCC8 Infographic

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In this infographic, you will see the following information for ABCC8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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